This study aimed at evaluating the effect of ractopamine (RAC) on metabolism, zootechnical performance, body composition, and behavior in Wistar rats submitted to acute and chronic restrain stress. The oral dose of 5 mg/kg of RAC was administered in periods of 0, 7, 14, 21, and 28 days. The elevated plus-maze test (EPMT) was used for behavioral assessment. Blood, carcass and viscera characteristics were evaluated. Insulin-dependent glucose transporters (GLUT-4) were semi-quantified by Western Blot in epididymal adipocytes. RAC periods associated with chronic stress increased the GLUT-4 protein expression in adipose tissue in a time-dependent manner (P=0.01), i.e., the longer the RAC addition period, the higher the GLUT-4 concentration in chronically stressed animals (0=1.42; 7=1.19; 14=2.03; 21=1.59; 28=2.35). The stress periods combined with RAC increased the time spent in the opened arms of the maze (Chronic stress: 0=10.6; 7=8.7; 14=5.9; 21=12.3; 28=4.0; Acute stress 0=3.1; 7= 4.7; 14=7.5; 21=0.0; 28=2.8) (P=0.04). Chronic (entries on the closed arms [ECA]=3.60) and acute (ECA=3.80) stress reduced locomotive activity in the maze (P=0.03). The results suggested that stress could negatively affect the possible benefits offered by the RAC, mainly impairing the adipose tissue metabolism and behavior in the animals.

The objective of this study was to evaluate the effect of photoperiod on locomotor activity, growth and gonadal development in Nile tilapia (Oreochromis niloticus) fingerlings. A completely randomised design was used, with five treatments (0L:24D, 6L:18D, 12L:12D, 18L:6D and 24L:0D) and four replicates, with the aquarium as the experimental unit. One hundred and sixty fingerlings of tilapia weighing 3.21±0.05 g and measuring 4.35±0.07 cm each were distributed among 20 aquaria of 20 L in a recirculation system with the temperature controlled to 27 °C, with eight fish per aquarium. Feeding was carried out twice daily for 75 days, with extruded feed containing 40% crude protein. The fingerlings subjected to a photoperiod of 12L:12D as well as those under 18L:6D and 24L:0D showed the greatest locomotor activity, whereas those under 6L:18D and 0L:24D showed the lowest activity. Fish subjected to a photoperiod of 18L:6D and 24L:0D showed the highest levels of performance. However, manipulation of the photoperiod did not influence the gonadal development, survival or the appearance of deformities in juvenile Nile tilapia. Under long photoperiods (18L:6D and 24L:0D), fish direct their energy to somatic growth and induce best feed efficiency.

The aim of this article was to evaluate the in vivo mechanism of action through which ractopamine reduces the amount of adipose tissue in carcass of animals fed diets supplemented with this drug. Literature search was carried out, using the keywords Ractopamine, lipogenesis, lipolysis, fatty acid and adipose tissue. The scoring system was designed for the adequacy of the methodology. A total of eight papers were found - all published in the English language. The determination of lipolysis and lipogenesis was carried out using different methods - from the quantification of non-esterified fatty acids in the blood to molecular analyses of adipose tissue. The review analysis suggested that, at least in the pigs, the use of this drug reduced lipid deposition in the carcass due to a greater inhibition of lipogenesis than an increase in lipolysis.